Effect of metallic deposited nanoparticles on screen printed carbon with tetrathiafulvalene incorporated ink, was tested by three electrochemical procedures. First one electrodeposition at constant potential and two cyclic voltammetry procedures that applied changes in step potential, scan rate and delay time. X-rays Fluorescence (XRF), Scanning Electronic and Atomic Force Microscopy (SEM) and (AFM) respectively were performed and metallic nanoparticles deposits were characterized through their SEM images. XRF provide metal percentage deposited by electrochemical procedures. AFM parameters let to know electrodes modi ied surface characteristics. Nanoparticles electrochemically deposited electrodes were modi ied with superoxide dismutase enzyme and tested on epinephrine biosensor amperometric calibration curves. Platinum Nanoparticles (PtNPs) modi ied electrodes nature surpasses AFM surface parameters. There is correlation between low AFM parameters and high slope epinephrine calibration curve excepting PtNPs case. Nanoparticles of platinum deposited by cyclic voltammetry procedure 1 showed higher epinephrine slope calibration curve than the others nanoparticles deposited by constant potential and cyclic voltammetry procedure. Higher linearity and stability was showed for PtNPs performed cyclic voltametry procedure; this one was selected to validation of biosensor. Developed biosensor showed reproducibility of 2.9% and detection limit of 22.8 μm. Biosensor analysis of epinephrine pharmaceutical injection was 103.1% with RSD 5.4%.
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