An amperometric biosensor based on Brassica napus hairy roots peroxidases to determine ochratoxin A is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (PBHR-Fc-CP) and is based on the fact that the decreased amount of H 2 O 2 produced by the action of PBHR is proportional to the oxidised amount of ochratoxin A in the solution. The PBHR- Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. The calibration curve was linear in the ochratoxin A concentration range from 1 x 10 -8 to 1,4 x 10 -4 mol L -1 . The lowest concentration value measured experimentally for a signal to noise ratio of 3:1 was 5.7 x 10-9 mol L -1 .
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