The time of a woman's life during pregnancy during which the circulatory system experiences hemodynamic and biochemical changes is known as pregnancy. There is a higher risk of developing chronic venous disease (CVD) at this time due to the remodelling of blood arteries and the exchange of maternal-fetal products. CVD may have long-term effects on both the mother and the baby after delivery. Previously, we looked into how pregnancy-associated CVD differs from healthy controls (HC) who have no history of the disease in terms of placental architecture at angiogenesis, lymphangiogenesis and villi morphology. Through the use of multiple markers, we wanted to more thoroughly explore the oxidative stress response in the placenta from women with CVD versus HC. A prospective, analytical and observational cohort study including 114 pregnant women was carried out (32 weeks). 62 patients in all had a clinical diagnosis of CVD. 52 healthy controls (HC) who had no prior history of CVD were also investigated. Real-time polymerase chain reaction and immunohistochemistry were used to examine the gene and protein expressions of NRF2, KEAP1, CUL3 and GSK-3. While Keap1, CUL-3 and GSK-3 gene and protein expressions were significantly reduced in the placental villi of women with CVD, Nrf2 gene and protein expressions were significantly higher. In the placenta of women with CVD, our findings identified abnormal gene and protein expression of Nrf2 and some of its primary regulators, Keap1, CUL-3 and GSK-3, which may be a sign of the oxidative environment seen in this tissue.
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