Mohamed A Zayed, Uzma Naim, Malik Darwech, Jaime Jung, Ronnie Eugea, Theresa Belgeri and Luis Sanchez
Washington University School of Medicine, USA
Veterans Affairs St. Louis Health Care System, USA
Scientific Tracks Abstracts: J Tissue Sci Eng
Objectives: Over three million Americans have advanced peripheral arterial occlusive disease leading to significant patient morbidity and mortality. The lack of well-preserved human peripheral arterial tissue substrate has limited scientific exploration of this disease process and development of impactful targeted molecular therapies. To address this, we developed an integrative biobanking strategy to collect peripheral arterial tissue specimens from patients undergoing vascular surgery. Methods: Over 22 months, we harvested vascular specimens from consenting patients undergoing open arterial endarterectomy and revascularization procedures. All patients were enrolled in an IRB approved protocol. A biobank infrastructure was developed to manage logistics, funding, collection, and real-time processing of harvested arterial tissue. Results: 356 patients were enrolled in the vascular surgery biobank prior to the index operation. 42 clinical variables were evaluated for each patient during the perioperative period. Vascular specimens were successfully collected for 54% (193) of patients who enrolled in the biobank. The majority of specimens collected were retrieved from the peripheral arterial system (50% carotid artery, 15% anterior or posterior tibial arteries, and 12% femoral or popliteal arteries). Each arterial specimen was sub-divided into maximally and minimally diseased portions to facilitate intra- and inter-patient biochemical and molecular analyses. Conclusions: An integrative biobanking approach in vascular surgery patients is feasible and provides a highly unique peripheral arterial substrate for molecular and biochemical analyses. Biobanking management and daily operations requires a dedicated team approach to insure proper patient consenting, specimen collection, and subsequent experimental analysis.
Mohamed A Zayed is a Surgeon-Scientist at Washington University School of Medicine, Department of Surgery and Section of Vascular Surgery. He has completed his medical training at Stanford University, and Doctoral degree in Pharmacology at the University of North Carolina at Chapel Hill. He has served as Chief Medical Officer for a software start-up company, and has published over 25 research articles in reputed journals. His current clinical and research interests focus on “The influences of diabetes on peripheral arterial disease”. This field provides a unique appreciation of the complexity and potential gaps of knowledge in vascular pathophysiology and its end-stage complications.
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