Use of highly 13C enriched biological samples and pure tracer substances for LC-HRMS based metabolomics

3^rd International Conference and Exhibition on Metabolomics & Systems Biology

March 24-26, 2014 Hilton San Antonio Airport, San Antonio, USA

R Schuhmacher, C Bueschl, B Kluger, N Neumann, A Simader, M Doppler, A Koutnik, M Lemmens, G Adam, AW Chassy, A Waterhouse and R Krska

Scientific Tracks Abstracts: Metabolomics

Abstract :

Stable isotope labeling assisted techniques show a high potential for being used in untargeted metabolomics studies. The presentation gives an introduction to the general concept and various applications of stable isotope labeling assisted LC- HRMS based metabolomics. Isotope techniques, recently established in the authors ́ laboratory will be presented including the preparation of highly stable isotope enriched filamentous fungi and plants. Moreover, the data processing concept and recently developed and refined MetExtractsoftware will be presented. Its use will be exemplified with a real-world dataset to illustrate the scope and power of the approach for the untargeted profiling of tracer metabolisation.