Usable nucleic acids from problematic sources

International Conference and Exhibition on Tissue Preservation & Bio-banking

July 20-22, 2015 Barcelona, Spain

Christian C Oste

Posters-Accepted Abstracts: J Tissue Sci Eng

Abstract :

The rapid advances made in personalized medicine are compelling the scientific and medical communities to gather an increasing volume of genetic/genomic information from an ever broadening diversity of sources. One of the major issues with this endeavor is the quality of the nucleic acids samples which can be obtained from the source material. Another one has to deal with how the purified nucleic acids are being conserved for future reference, which in itself would be the topic of another presentation. This presentation will focus on two particular cases, whose handling requires some precaution in order to safely obtain usable nucleic acids. The first case deals with the purification of sequence-able nucleic acids from paraffin-embedded tissues (FFPE). An automated protocol will be described, from starting sample to DNA ready to be sequenced. The second case addresses more specifically the purification of DNA samples from large volume, complex and heterogeneous samples, such as in the cases of microbiome and metagenome analysis. The automated protocols which are being developed for these applications will be described as well.