Tissue preservation, quality controls and aliquoting methods in biobanking

2^nd International Conference & Exhibition on Tissue preservation and Bio-banking

September 12-13, 2016 Philadelphia, USA

Vincenzo Canzonieri, Silvia Cervo, Tiziana Perin, Paolo De Paoli and Agostino Steffan

CRO Aviano National Cancer Institute, Italy

Posters & Accepted Abstracts: J Tissue Sci Eng

Abstract :

CRO-Biobank (the Biobank of the National Cancer Institute CRO Aviano) is a structured facility integrated in a clinical setting aimed at collecting human biological samples for cancer research. Our mission is to organize a collection of frozen and Formalin Fixed Paraffin Embedded (FFPE) tissue samples with associated biomaterials (e.g. serum, plasma, buffy coat, and nucleic acid). Frozen tissue samples are OCT-embedded, flash frozen in liquid nitrogen and stored at -80�?°C. For clinical diagnosis, molecular analysis and biobanking of paraffin-embedded tissues, we are developing a specific experience on different histological fixatives, alternative to formalin, which is toxic and carcinogenic. We use well-defined methods to control tissue quality: Histocytology (frozen sections, FFPE mirrored samples and cytological imprint) and molecular pathology. As quality control of DNA, RNA and protein extracts, we randomly perform molecular analyses using appropriate positive and negative controls. Based on the model of â�?�?Expert Centersâ�?, a specific activity of biological characterization of solid and hematological tumors has been planned in our Institution, using aliquots of samples stored in our biobank. NGS, proteomic and immunohistochemical preliminary analyses will provide selected series of highly informative biosamples to the researchers. In order to optimize the use of biological samples, we have elaborated an â�?�?aliquoting sytemâ�? that allows to select the appropriate quantity of material delivered for a single project and may warrant appropriate selection of cells for research purposes. Moreover, we are implementing quality processes and procedures to guarantee the safety and privacy of the providers and to ensure both the traceability and the quality of samples. We also consider ethical, legal, social issues (ELSI) and IT topics, taking into account indications from the Common Service ELSI established in 2015, as a key asset of BBMRI-ERIC.

Biography :

Email: vcanzonieri@cro.it