Standardization of indirect ELISA for antibody detection to PPR in vaccinated sheep

2^nd Indo-Global Summit & Expo on Veterinary

October 26-28, 2015 Hyderabad, India

Kavitha Kandimalla and Dhanalakshmi K

Sri P.V. Narasimha Rao Telangana State University for Veterinary, Animal and Fishery Sciences, India

Posters-Accepted Abstracts: J Vet Sci Technol

Abstract :

Peste Des Petits Ruminants (PPR) is an acute, highly contagious, economically important disease of small ruminants with high rates of morbidity and mortality. Clinically the disease resembles Rinderpest and is characterized by severe pyrexia, occulonasal discharges, pneumonia, necrotizing and erosive stomatitis and enteritis leading to severe diarrhea. As the incidence of RP went down PPR has taken a rising toll. Andhra Pradesh state has become the centre of major epidemics of PPR. There is no suitable test for detection of PPRV antibodies at field level at an affordable price. Serum Neutralization Test and c-ELISA are commonly employed diagnostic techniques for testing serum samples. Most of the state laboratories cannot afford screening of large number of samples by SNT as it is a laborious, time consuming and requires cell culture facilities. The use of monoclonal antibodies in c-ELISA makes it economically non viable under field conditions when compared to indirect ELISA which is much cheaper as it does not require the use of any monoclonal antibodies. In view of the above limitations, the present study was taken up to develop a polyclonal based indirect ELISA for detection of antibodies to PPRV using partially purified PPR viral antigen propagated in Vero cell lines. A cut-off value (0.627) was set as twice the mean (0.5127) of negative population based on the distribution of known negative serum samples (N=50) in respect of PPR viral antibodies in the test. For validation of assay, a total of 500 sera samples were tested. The efficacy of the present assay compared very well with SNT having high relative specificity (100%) and relatively low sensitivity (84%) and can be used as a good alternative to c-ELISA and SNT for disease monitoring and sero surveillance.

Biography :

Email: drkkreddy2000@yahoo.com