GET THE APP

Rediscovered CD8 Treg
..

Molecular and Genetic Medicine

ISSN: 1747-0862

Open Access

Rediscovered CD8 Treg


Joint Meeting on 4th World Congress on Human Genetics & Genetic Diseases and 3rd International Conference on Molecular Medicine & Diagnostics

April 19-20, 2018 Dubai, UAE

Sudhir Gupta

University of California, USA

Posters & Accepted Abstracts: J Mol Genet Med

Abstract :

Recently CD8+ T cells have been demonstrated to regulate proliferation of CD8+ T cells. We have investigated a role of CD8+ T cells in regulating various functions of autologous CD4+ T cells. Activated CD8+ T cells, in a concentration-dependent manner, inhibited cell division (CFSE dilution) and DNA synthesis (3H thymidine incorporation) of autologous CD4+ T cells. CD8+ T cells also inhibit differentiation of Na�¯ve CD4+ to effector memory CD4+ (TEM) and CD45RA+ terminally differentiated effector memory/exhausted CD4+ T cells (TEMRA). Activated CD8+ T cells mediated their regulatory effect, at least in part, by soluble mediator (s) and not due to direct cytotoxicity/apoptosis of CD4+ T cells. Activated CD8+ T cells secrete predominantly IL-10 and MIP1�² and inhibition of CD4+ T cell proliferation by activated CD8+ T cells is significantly blocked by neutralizing antibodies against IL-10 and MIP1�², but not by antibodies against TGF�² or PD-1. CD8+ Treg cells inhibit secretion of IFN-�³ and IL-17A by autologous CD4+ T cells. CD8+ CXCR3+ CCR7+CD45RA- (CD183+) sorted cells mediated majority of the regulatory activity of activated CD8+ T cells. CD8 Treg were further characterized phenotypically for FoxP3+ expression and appears to be of CD8+CCR7+CD25hiICOS+CTLA-4+FoxP3+ phenotype. Furthermore, proportion of CD8 Treg (CD8+CD183+CCR7+CD45RA-FoxP3+) in aged humans is comparable to young subjects; however, they are impaired in their regulatory functions. In primary immunodeficiency, both quantitative and qualitative defects in CD8 Treg may explain increased susceptibility to autoimmune diseases. sgupta@uci.edu

Google Scholar citation report
Citations: 3919

Molecular and Genetic Medicine received 3919 citations as per Google Scholar report

Molecular and Genetic Medicine peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward