Ahmad I Funjan*
Traditional identification methods for the soft rot Erwinias are both imprecise and time-consuming. We have used the 16S rDNA to aid in their identification.
Analysis of 16S rDNA-PCR and 16S rDNA-RFLP and gene sequencing was found to be simple, precise, and rapid method compared to other molecular techniques.
Analysis of the isolates genome by using their total DNA by amplifying their genome using the universal primer (fD1/rP2) indicated an amplified product of the
16S rDNA at 450 bp and the amplification using the specific 16S rDNA (EP16A/EP1GTC) was located of 700 bp. The restriction analysis of the universal amplified
product of 450 bp size using Hind III, proved the presence of different RFLP bands with some common bands. Variation in RFLP profile bands is an indication of
polymorphism in between the isolates. Whereas common bands indicates genetic stability among the isolates of the same spices or same genus. Sequencing of
the 16S rDNA universal primer amplified product showed a complete sequence of one isolate out of 10 isolate and the similarity between this isolate and the data
base indicated the presence of high similarity above 95% with other Enterobaceriaceae.
DOI: 10.37421/1747-0862.2023.17.637
Cardio-Vascular Disease (CVD) is a leading cause of death worldwide. The development of CVD is influenced by both environmental and genetic factors. Recent advancements in genomic technologies have allowed for the identification of genetic variants that are associated with increased risk of CVD. In this paper, we will review the current literature on the role of genetic variants in the development of CVD. We will discuss the various genetic variants that have been identified and their mechanisms of action. Furthermore, we will explore the potential clinical applications of genetic testing in predicting CVD risk and tailoring treatment strategies.
Tatiana Muratore
DOI: 10.37421/1747-0862.2024.18.664
Neuroblastoma is a common pediatric cancer originating from neural crest cells. Among its various genetic mutations, the Anaplastic Lymphoma Kinase (ALK) gene mutations are significant due to their role in tumorigenesis and therapy resistance. This article presents a case of an infant with a germline ALK F1174I mutation, multifocal neuroblastoma, and central hypoventilation, providing insights into the clinical, genetic, and therapeutic implications of this mutation. Through a detailed case study, literature review, and discussion, we aim to elucidate the pathophysiology and potential treatment strategies for this rare presentation, highlighting the need for personalized medicine in pediatric oncology.
Windy Saultier
DOI: 10.37421/1747-0862.2024.18.670
Anna Lavrov*
Gene therapy represents one of the most revolutionary advancements in modern medicine, holding the promise to not only treat but potentially cure a range of genetic disorders that were previously considered untreatable. The concept of gene therapy revolves around the introduction, alteration, or removal of genetic material within a person’s cells to treat or prevent disease. The potential benefits of this innovative approach have captured the imagination of both scientists and patients alike, as it moves from the realm of theoretical possibilities into tangible clinical applications. As this technology continues to evolve, it is on the cusp of reshaping how we approach treatment for genetic diseases, with profound implications for the future of medicine.
Serban Riza*
Molecular genetic testing is a revolutionary tool that has transformed the way we understand and approach the diagnosis and management of various diseases. By analyzing DNA, RNA, and proteins, molecular genetic testing can identify genetic mutations, alterations, or predispositions that may contribute to the onset of diseases. With advancements in molecular biology and technology, these tests are increasingly being utilized for early disease detection, providing the opportunity for early intervention and personalized treatment plans. This manuscript delves into the significance of molecular genetic testing, its applications in disease detection, its impact on healthcare, and its potential for the future of medicine.
Zhou Guo*
In the ever-evolving field of medical diagnostics, the integration of molecular techniques has brought about transformative changes, revolutionizing the way healthcare professionals detect, diagnose, and monitor diseases. Over the past few decades, advancements in molecular biology, genomics, and biotechnology have significantly enhanced our understanding of disease mechanisms at a molecular level, providing more precise and personalized diagnostic tools. These molecular techniques, which include Polymerase Chain Reaction (PCR), Next-Generation Sequencing (NGS), microarray analysis, and various types of biomarkers, have had a profound impact on how we approach the diagnosis of both infectious and non-infectious diseases, genetic disorders, cancers, and even rare conditions. This manuscript explores the major contributions of molecular techniques to medical diagnostics, their applications, and the future potential they hold in transforming healthcare.
MacÃas Fraga*
Metabolomics, the scientific study of metabolites and their roles within biological systems, has rapidly evolved as a powerful tool in the field of personalized medicine. Metabolites are the small molecules that are the end products of cellular processes and provide crucial insights into the biochemical state of an organism. They are directly linked to an individual’s health and disease status, making them a promising biomarker for disease detection, prognosis, and treatment response. Personalized medicine, also known as precision medicine, is an approach to medical treatment and care that takes into account the individual’s genetic makeup, environment, and lifestyle. By using this tailored approach, personalized medicine aims to provide more effective treatments with fewer side effects. In this context, metabolomics plays a key role in enhancing the precision and accuracy of medical interventions.
Sun Yoon*
Aging is a complex biological process influenced by various factors, including genetics, environmental exposures, and lifestyle choices. As we age, our bodies undergo numerous physiological changes that often lead to a decline in functional capacity and the onset of age-related diseases. One area of growing interest in the scientific community is the potential role of bioactive compounds in mitigating the negative effects of aging. Bioactive compounds, which are naturally occurring chemicals found in foods, plants, and other natural sources, have been shown to have beneficial effects on human health. These compounds are capable of influencing cellular functions, modulating biological pathways, and reducing oxidative stress, inflammation, and cellular damage, all of which are closely associated with the aging process.
Roberto Magro*
Gene therapy has evolved significantly over the past few decades, transforming from a theoretical concept to a promising clinical reality that has the potential to revolutionize medicine. The journey of gene therapy is one of continuous scientific discovery, innovation, challenges, and breakthroughs. It has required not only advancements in molecular biology and genetics but also improvements in the technology needed to deliver therapeutic genes to the human body safely and effectively. This journey, from research to reality, is deeply intertwined with the broader history of biotechnology and genetic research, where initial aspirations of altering genes to treat diseases faced numerous hurdles. However, as technology has advanced and our understanding of genetics deepened, gene therapy has moved closer to providing viable solutions for a wide range of genetic disorders.
Rushing Vaidota*
Precision medicine represents a transformative approach to healthcare that tailors treatment strategies to individual patients based on their unique genetic, environmental, and lifestyle factors. This personalized model contrasts with the traditional “one-size-fits-all” method, which often results in treatments that are not optimized for every patient. One of the essential emerging fields that are significantly contributing to the advancement of precision medicine is metabolomics. Metabolomics involves the study of metabolites, which are the small molecules involved in the chemical processes within and related to living cells. By offering insights into the metabolic status of an individual, metabolomics can aid in the identification of biomarkers, provide a deeper understanding of disease mechanisms, and help to optimize treatment regimens for individual patients.
Bahhou Amine*
Molecular diagnosis has revolutionized modern medicine, providing healthcare professionals with unprecedented insights into the underlying mechanisms of disease. This transformative approach to diagnostics transcends the traditional methods of physical examination, blood tests, and imaging techniques by focusing on the genetic and molecular levels of disease. As science has advanced, particularly in the fields of genomics, proteomics, and bioinformatics, molecular diagnosis has become an integral part of medical practice, enabling clinicians to make more accurate diagnoses, tailor personalized treatment plans, and predict patient outcomes with greater precision.
Molecular and Genetic Medicine received 3919 citations as per Google Scholar report
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