Molecular and Genetic Medicine

Articles in press and Articles in process

Research Article Pages: 1 - 8


Molecular Analysis of Erwinia Species Based on 16S rDNA Gene Sequences

Ahmad I Funjan*

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Traditional identification methods for the soft rot Erwinias are both imprecise and time-consuming. We have used the 16S rDNA to aid in their identification.
Analysis of 16S rDNA-PCR and 16S rDNA-RFLP and gene sequencing was found to be simple, precise, and rapid method compared to other molecular techniques.
Analysis of the isolates genome by using their total DNA by amplifying their genome using the universal primer (fD1/rP2) indicated an amplified product of the
16S rDNA at 450 bp and the amplification using the specific 16S rDNA (EP16A/EP1GTC) was located of 700 bp. The restriction analysis of the universal amplified
product of 450 bp size using Hind III, proved the presence of different RFLP bands with some common bands. Variation in RFLP profile bands is an indication of
polymorphism in between the isolates. Whereas common bands indicates genetic stability among the isolates of the same spices or same genus. Sequencing of
the 16S rDNA universal primer amplified product showed a complete sequence of one isolate out of 10 isolate and the similarity between this isolate and the data
base indicated the presence of high similarity above 95% with other Enterobaceriaceae.

Perspective Pages: 1 - 2


Investigating the Role of Genetic Variants in the Development of Cardiovascular Disease

Edwin Eliana*

DOI: 10.37421/1747-0862.2023.17.637

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Cardio-Vascular Disease (CVD) is a leading cause of death worldwide. The development of CVD is influenced by both environmental and genetic factors. Recent advancements in genomic technologies have allowed for the identification of genetic variants that are associated with increased risk of CVD. In this paper, we will review the current literature on the role of genetic variants in the development of CVD. We will discuss the various genetic variants that have been identified and their mechanisms of action. Furthermore, we will explore the potential clinical applications of genetic testing in predicting CVD risk and tailoring treatment strategies.