Plasmid-mediated antimicrobial resistances of V. parahaemolyticus from pacific white shrimp and E. coli from diarrheal swine

International Conference on Medical and Clinical Microbiology

July 03-04, 2017 Bangkok, Thailand

Patamabhorn Amavisit, Chea Rortana, Worawidh Wajjwalku, Visanu Boonyawiwat, Charuwan Hrianpreecha and Suksan Chamsing

Kasetsart University, Thailand

Scientific Tracks Abstracts: J Med Microb Diagn

Abstract :

Vibrio parahaemolyticus isolated from the Pacific white shrimp (Litopenaeus vannamei) and Escherichia coli isolated from intestinal organs of diarrhea swine were tested antimicrobial sensitivity and studied on plasmid-mediated antimicrobial resistance genes. The antimicrobial resistant (AMR) rates of V. parahaemolyticus were 98.48% ampicillin, 3.03% doxycycline, 4.55% oxytetracycline, 6.06% erythromycin, 1.52% florfenicol, and 1.52% sulphamethoxazole-trimetroprime. V. parahaemolyticus were not resistant to tested quinolone agents (ciprofloxacin, enrofloxacin, norfloxacin and ofloxacin), but some isolates presented intermediate susceptibility of the agents. Plasmid-mediated quinolone resistance (PMQR) gene, qnrVC was found in only one isolate. DNA amplification of pirAB-like genes, which caused acute hepatopancreatic necrosis disease (AHPND) in shrimp, showed that 39.39% of V. parahaemolyticus carried this virulent gene. However acquisition of pirAB-like gene virulent factor in V. parahaemolyticus was not related to their AMR. While the AMR rates of E. coli were relatively high at 98.41% amoxicillin, 98.41% ampicillin, 96.83% cephalexin, 69.84% ciprofloxacin, 69.84% enrofloxacin, 57.14% fosfomycin, 77.78% colistin, 84.13% gentamicin, and 90.48% oxytetracycline. The rates of E. coli carried PMQR genes were 61% of qnrS and 9.5% of oqxA. Colistin resistant gene mcr-1 that located on plasmid was also amplified and found that 25.40% of the isolates carried mcr-1 gene and they had colistin MIC 2 �¼g/mL.

Biography :

Patamabhorn Amavisit is an associate professor who is working for the Faculty of Veterinary Medicine, Kasetsart University, Thailand. Her researches focus on veterinary microbiology including foodborne bacteria isolated from animal sources and antimicrobial resistant (AMR) bacteria. Since the launch of WHO global action plan on AMR, the researches have been performed for a part of policy decision in the country level using One Health aspects involving the surveillance of AMR contamination in particular bacteria contaminated in animals and environment.

Email: fvetpaa@ku.ac.th