Mutation detection of RB1 tumor suppressor gene (exon 18 and 19) and expression assay of FGF2 oncogene in patients with brain tumors including meningioma and astrocytoma

Molecular and Genetic Medicine

ISSN: 1747-0862

Open Access

Mutation detection of RB1 tumor suppressor gene (exon 18 & 19) and expression assay of FGF2 oncogene in patients with brain tumors including meningioma and astrocytoma

6th International Conference on Genomics & Pharmacogenomics

September 12-14, 2016 Berlin, Germany

Ali Hosseini Bereshneh, Ellahe Abdollahi, Mohammad Roushanghalb, Saeed Tarverdizadeh, Reyhane Sadat Saeedi and Parvin Mehdipor

Tehran University of Medical Sciences, Iran

Scientific Tracks Abstracts: J Mol Genet Med

Abstract :

Most studies have shown that there are association between the development and malignancy of brain tumors and tumor suppressor genes and oncogenes. The aim of this project is to investigate the RB1 gene mutations in exon 18 and 19 and FGF2 oncogene expression in patients with Astrocytoma and Meningioma type├ó┬?┬?s brain tumor. This is an in vitro study in which the extraction of DNA from 20 samples of fresh brain tissue was performed by phenol-chloroform protocols. After PCR amplification of exon 18 and 19 of RB1 gene, screening by SSCP (Single-Strand Conformation Polymorphism) was performed to detect the possible shifts. Those shifts were sequenced. Then the cells were extracted from fresh brain tissue for analysis of FGF2 gene expression by flow cytometry. In this study was detected two unknown variation (Heterozygote Substitution) in intronic regions including c.1815- 170T>G and c.1960+107T>G; and one insertion mutation in intronic region c.1960+108_1960+109isnT. It was observed that FGF2 gene expression in astrocytoma samples was increased. Findings from this study were indicated that mutations in RB1 gene mostly was in malignant astrocytoma brain tumor type; also, since the FGF2 gene expression in astrocytoma brain tumors was higher than benign meningioma type of brain tumors. Overexpression of FGF2 gene may play an essential role in malignant brain tumor and this may be important in diagnosis and new therapeutic methods of brain tumors treatment.

Biography :

Ali Hosseini Bereshneh has completed his Bsc in Clinical Laboratory Sciences (Medical Technology) from Mashhad University of Medical Sciences and he is currently pursuing MSc in Human Genetics, Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences. He has published more than 15 national and international papers in Iranian and international journals. He has published 5 national books in the area of Medical Genetics, Genetics of Dentistry and Prenatal Diagnosis & Genetic Counseling. He is a Member of Iranian Medical Genetics Society and Medical Laboratory Society.


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