P.A. Hertzen Moscow Cancer Research Institute, Russia
Posters-Accepted Abstracts: J Cytol Histol
The aim of our study was to determine the possibilities of cytological method in combination with the method of flow cytometry in the diagnosis of lymphoma and its immunophenotyping. Material for the study served as a fine-needle aspiration biopsy of the lymph nodes of the patient 243 made under the control of ultrasound. Some cellular material stained with azure-eosin mixtures and subjected to routine cytology. Most of the cellular material was used for flow cytometry and immunocytochemistry. Immunophenotyping was performed by flow cytometry FACS Calibur firm Becton Dikinson, USA. Cell suspensions were stained with antibodies 2- and 3-color fluorescent markers. For tipirovpaniya lymphomas using the following panel of antibodies: CD3, CD4, CD5, CD7, CD8, CD10, CD15, CD19, CD20, CD21, CD23, CD30, CD34, CD38, CD43, CD45, CD56, CD57, CD79a, CD138, HLA- DR, FMC-7, TdT, immunoglobulin light chain kappa and lambda. Immunocytochemistry was performed by Ultra Vision using antibodies: bcl-2, Cyclin D1, Ki-67, EMA. Total contact cytologically diagnosed with non-Hodgkin├ó┬?┬?s lymphoma in 243 patients. Carrying immunophenotyping by flow cytometry possible to determine B-cell lymphoma in 228 cases: 90 of them-follicular lymphoma, 17-B-cell lymphoma of the small lymphocytes, 6-mantle cell lymphoma, diffuse large 112 B-cell lymphomas, 3-MALT-lymphoma. T-cell lymphoma was observed in 15 patients of whom at 8-cell lymphoma with immunophenotype of peripheral T lymphocytes, 7-anaplastic large cell lymphoma). The combination of cytology methods with flow cytometry and immunocytochemistry can not only quickly and safe for the patient to establish the diagnosis of lymphoma (accuracy-98%) but its immunophenotype defining further treatment and prognosis of the disease (accuracy-90%).
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