It can be defined as the process of antigen or protein detection in the biological sample using antigen specific antibodies. Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors.
There are two types of immunohistochemical staining: direct and indirect method. The direct method is a single step staining method and involves a labeled antibody reacting directly with the antigen in tissue sections. In indirect method the unlabeled primary antibody (first layer) binds to the target antigen in the tissue sample and then a labelled secondary antibody (second layer) is added that reacts with the primary antibody
Related Journals of Immuno-cytochemistry
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