Isolation, diversity and biotechnological potential of rhizo- and endophytic-bacteria associated with mangroves plants from Saudi Arabia

JOINT EVENT ON 15^th International PHARMACEUTICAL MICROBIOLOGY AND BIOTECHNOLOGY CONFERENCE & 10^th Annual MEDICAL MICROBIOLOGY SUMMIT & EXPO

June 21-23, 2017 London, UK

Fehmida Bibi, Sana Akhtar Alvi, Muhammad Yasir and Esam I Azhar

King Abdulaziz University, Saudi Arabia

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Marine bacteria from decades are an exceptional source of halotolerant enzymes. The aim of the present study was to isolate hydrolytic enzyme producing bacteria from seven different mangroves collected from the coastal area of Thuwal, Jeddah, Saudi Arabia and to further screen them for other enzymatic and antifungal activities. We have isolated 46 different enzymatic rhizo and endophytic bacteria from soil, roots and leaves of the mangroves using different enzymatic media. These bacterial strains were capable of producing industrially important enzyme (cellulase, protease, lipase, and amylase). Bacteria were screened further for antagonistic activity against fungal pathogens. Finally these bacterial strains were identified on the basis of 16S rRNA gene sequences. Taxonomic and phylogenetic analysis revealed 95.9-100% sequence identity to type strains of related species. The dominant phylum was gammaproteobacteria (�³-Proteobacteria), comprised of 10 different genera; Erwinia, Vibrio, Psychrobacter, Aidingimonas, Marinobacter, Chromohalobacter, Halomonas, Microbulbifer, and Alteromonas. Firmicutes was second dominant phylum comprised of only one genus Bacillus. Similarly, only 1 genus Isoptericola belongs to Actinobacteria was identified. Further, these enzymatic bacteria were tested for other enzymes production. Most of the active strains showed cellulytic and lipolytic activities. Many of them were also active against fungal pathogens tested. Our results demonstrated that the mangroves represent an important source of potentially active bacteria producing enzyme and antifungal metabolites. It is concluded from this study that mangroves harbor diverse group of bacteria and their bioactive products. These bacteria are source of novel halophilic enzymes and antibiotics of industrial and medicinal use.

Biography :

Email: mimrannaseer@yahoo.com