Isolation and characterization of amicoumacins produced by Bacillus pumilus 35R COPS isolated from Acartia tonsa copepod antagonistic agents against pathogenic strains

47^th World Congress on Microbiology

September 10-11, 2018 | London, UK

Mahammed Zidour

Univ. Artois, INRA, ISA, Univ. Lille, Univ. Littoral Cote d Opale, EA 7394 - ICV – Institut Charles Viollette, F-59000 Lille, France

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

A marine bacterium named 35R COPS was isolated from Acartia tonsa copepod eggs. The isolate was identified as Bacillus pumilus by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and by amplification and sequencing of the 16S rRNA gene followed by a phylogenetic analysis. The B. pumilus 35R COPS is highly inhibitory against several pathogens, including Vibrio angularum, Vibrio alginolyticus and Staphylococcus aureus (MRSA). The antimicrobial activity of the 35R COPS strain is mainly related to the production of several compounds of the amicoumacin family such as amicoumacin A, amicoumacin B, phosphoamicoumacin A and phosphoamicoumacin B. The Amicoumacin structures were elucidated using their specific UV absorption profiles and their MS/MS fragmentation patterns. Genomic sequencing of the B. pumilus 35R COPS strain associated with its bioinformatics analysis show the existence of a gene cluster coding for amicoumacin. This bacterium may be a promising candidate strain as probiotic in aquaculture and as agents for the prevention of various animals and plant infections. The assessment of the cellular toxicity are ongoing.

Biography :

E-mail: mahamed.zidour@hotmail.com