Jaypee University, India
Posters & Accepted Abstracts: Pharmacoeconomics
Polyphenon 60 (P60) and curcumin (CUR) were loaded in a single nano emulsion system and their combined antibacterial action was studied against uropathogenic Escherichia coli. To enhance bioavailability at target organs and to inhibit enzymatic degradation in gastric intestinal tract, vaginal route of administration was explored. P60+CUR nanoemulsion (NE) was formulated by ultra-sonication and optimized using Box-Behnken design. Optimized NE showed Z-average of 211.2 nm, polydispersity index 0.343 and zeta potential -32.7 mV. Optimized P60+CUR NE was characterized for stability and transmission electron microscopy (TEM) and it was observed that NE was stable at 4├?┬║C for 30 days and monodispersed in nature with particle size in the range of 195-205 nm. P60+CUR NE was further formulated to gel and characterized by viscosity parameters and in-vitro permeation studies. In vitro drug release studies in simulated vaginal media showed maximum release (84├?┬▒0.21%) of curcumin within 5 h and (91├?┬▒0.16%) of P60 within 8 h. Both the drugs maintained sustainable release up to 12 h. To investigate the transport via intravaginal route, gamma scintigraphy and biodistribution study of P60+CUR NBG was performed on Sprague-Dawley rats using 99m technetium pertechnetate for radiolabeling to P60 molecule. Following intravaginal administration, P60+CUR NBG dispersed in kidney and urinary bladder with (3.07├?┬▒0.15) and (3.35├?┬▒0.45) percentage per gram after 3 h, respectively and remained active for 12 h. Scintigraphy images and biodistribution findings suggested that the P60+CUR NBG given by intravaginal route led to effective distribution of actives in urinary tract to eradicate urinary tract infection.
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