FSH and LH can be substituted by eCG in maturation media for caprine embryo or parthenote production in vitro

7^th International Veterinary Congress

September 04-05, 2017 | Paris, France

Justin Kouamo and Suresh Drinker Kharche

University of Ngaoundere, Cameroon
CIRG, India

Posters & Accepted Abstracts: J Vet Sci Technol

Abstract :

In most of the mammalian IVM (in vitro maturation), the basic medium is supplemented with serum and hormones. FSH (follicle stimulating hormone) and LH (luteinizing hormone) are generally used in in vitro maturation protocols to improve fertilization, early embryo development and cumulus expansion. Commercial eCG (equine chorionic gonadotrophin) is less expensive than FSH and LH. This study aimed to evaluate the substitution of FSH and LH by eCG on in vitro maturation, in vitro fertilization and in vitro embryo or parthenote development goat oocytes. Three experiments were performed: the first was to evaluate the dose effect of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin on in vitro maturation goat oocytes; the second was to compare the embryo development from caprine oocytes matured in a medium supplemented with eCG alone or in combination with hCG (human chorionic gonadotropin); the third was to compare the parthenogenetic activation and in vitro fertilization of in vitro matured caprine oocytes. The results indicated that: 1. Supplementation of 20 IU/mL eCG in maturation media significantly (P<0.05) increased maturation rate which was further enhanced by addition of 20IU hCG and may be used in in vitro maturation protocols. 2. Oocytes matured with 20IU/ ml eCG had a good cytoplasmic maturation that allows normal embryo development up to blastocyst stage without addition of hCG. Thus, PMSG at 20IU/ml in maturation medium can be used to reduce the cost of in vitro embryo production. 3. The cleavage rate from caprine oocytes matured in a medium supplemented with 20IU/ml eCG was comparatively higher following parthenogenetic activation with ionomycin/6-DMAP (6-dimethylaminopurine) than IVF (in vitro fertilization).