Formulation, stability studies and analysis of new ayurvedic health supplement for babies

2^nd International Conference and Exhibition on Traditional & Alternative Medicine

August 25-26, 2014 DoubleTree by Hilton Beijing, China

Deepa Padmaja

Accepted Abstracts: Altern Integ Med

Abstract :

With the emergence of antibiotic-resistant Staph. sp., searching for antimicrobial agents other than antibiotic is of great concern. The study aimed to determine both MIC & MBC of different honey samples against these strains since it was conducted with 64 Staph sp. recovered from bovine mastitis which were tested in vitro against 11 antimicrobial agents. The most multidrug resistant (MDR) strains (n. 19) representing the four species; S. aureus (n. 6), S. intermediu s (n. 3); S. epidermidis (n. 5) and S. saprophyticus (n. 5) were tested against six honey batches; marjoram, cotton, two different fennel samples and two different trefoil samples as well as they were tested against 10% w/v propolis-fennel honey mixture. Both MIC & MBC of the six honey samples and propolis-honey mixture against every tested strain were determined. The study concluded that all tested bacterial strains, despite of being MDR- were sensitive to the antimicrobial activity of all tested honeys but differently. Against all Staph sp., trefoil honey batch-2 had the lowest MIC value (20.83% v/v) but cotton had the lowest MBC one (37.92% v/v) among the six tested honey batches without any significant differences, while 10% w/v propolis-fennel honey mixture showed the lowest both MIC & MBC values against all Staph sp. As a whole, the study had (13.96 & 28.26% v/v respectively) high significant differences (p>0.01). Against different Staph sp., it was found that propolis honey mixture had the lowest MIC value against S. intermediu s followed by S. aureus as 6.2 & 7.25% v/v respectively with high significant differences (p>0.01), and MBC values as 12.5 & 14.58% respectively. Among the different Staph. sp., S. aureus was the most sensitive species to the honey antimicrobial action since MIC & MBC as 13.3 & 27.1% v/v respectively with highly significant differences (P>0.01).