Exhaled breath condensates-source of biomarkers of respiratory disease in animals

2^nd Indo-Global Summit & Expo on Veterinary

October 26-28, 2015 Hyderabad, India

Umesh Balaji Sontakke

ICAR-National Dairy Research Institute, India

Posters-Accepted Abstracts: J Vet Sci Technol

Abstract :

Respiratory diseases are major problem for dairy producers. These diseases are commonly associated with calves and dairy cows. Exhaled breath condensate (EBC) is a source of biomarkers of lung disease. EBC is an exhalate from breath which is condensed by cooling using a collection device. Exhaled breath consist of volatile substances such as carbon dioxide, nitric oxide, ethane, pentane and non-volatile compounds such as hydrogen peroxide, eicosanoids, cytokines, electrolytes and water vapor and bio-aerosols (bacteria, viruses, fungi) that derive from the respiratory tract. Concentrations of these substances are influenced by lung diseases and modulated by therapeutics treatment. Aldehydes like hexanal, heptanal are lipid peroxides their concentration in the EBC indicates oxidant induced damage. The oxidative biomarkers like H2O2 & NO are present in the exhaled breath and their concentration indicates the lung condition. H2O2 is released from activated inflammatory cells during respiratory burst. Nitric oxide (NO) originates from endogenous sources such as alveoli, airway epithelium and inflammatory cells. PH of EBC also changes in respiratory diseases. EBC can be performed in conscious as well as anesthetized animals. EBC is a non-invasive method, simple to perform, can be repeated frequently. The invasive methods commonly used to analyze immune parameter and inflammatory mediators in the lung of animals are like bronchoalveolar lavage, lung biopsy. EBC method is one of the diagnostic tool usually practice in humans for lower respiratory tract diseases but only few studies available in veterinary medicine. EBC can be collected successfully in animals but interpretation of results is difficult due to the wide range of the concentration of inflammatory markers in healthy animals. Future work is necessary to determine normal ranges for values of inflammatory markers in large number of different animals and to investigate change associated with lung diseases.

Biography :

Email: umeshndri@gmail.com