Effects of TRPC6 on invasibility of low-differentiated prostate cancer cells

Journal of Metabolic Syndrome

ISSN: 2167-0943

Open Access

Effects of TRPC6 on invasibility of low-differentiated prostate cancer cells

International Conference on Metabolic Syndromes

October 17-18, 2016 Rome, Italy

Wang Dong

Sichuan Provincial People's Hospital, China

Posters & Accepted Abstracts: J Metabolic Synd

Abstract :

Objective: To study the expression of transient receptor potential cation channel (TRPC6) among prostate cancer (PCA) cells, establish high expression cell lines of TRPC6 and to provide potential cell mode for PCA oncogenesis and development. Methods: Occurrence and development of PCA cells, PC3, PC-3M, DU145, 22 rv1, LNCaP and normal prostate epithelial cells in the PrEC TRPC6 expression level were detected by QPCR. Calcium phosphate transfection was used to package retrovirus pLEGFP-N1-TRPC6 and pLEGFP-N1-vector and infect the PCA cells, a stable high expression of TRPC6 PCA cells. Sable cell lines of TRPC6, MMP-2, MMP-9 expression was detected by QPCR and Western blot. Change of cell invasion ability was detected by Transwell. Results: The expression level of PCA cells TRPC6 were higher than control group PrEC cells. Among TPRC6, the expression of cell line PC3 transfer potential were the lowest, and high transfer cell line PC-3M expression was the highest. RT-PCR and Western blot results showed that after filter, the seventh generation of cell TRPC6 protein and mRNA expression levels were higher than the control group. Transwell experimental results showed that the overexpression of TRPC6 could promote the invasion ability of PC3 PCA cells. Conclusions: TRPC6 expressed in PCA cells is in disorder, and its action may be associated with the invasion and metastasis of PCA cells; successful establishment of stable high expression of TRPC6 PCA cells primarily confirm the invasion-trigger ability of TRPC6 on PCA, and lay down the foundation for exploring the TRPC6's role in the occurrence and development of PCA mechanism.

Biography :


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Citations: 48

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