Development of PCR-based detection and quantification of animal fibers from textile materials

2^nd Indo-Global Summit & Expo on Veterinary

October 26-28, 2015 Hyderabad, India

Rajiv Kumar, Satish Kumar and S M K Naqvi

ICAR-Central Sheep and Wool Research Institute, India

Posters-Accepted Abstracts: J Vet Sci Technol

Abstract :

There is worldwide demand of animal specialty fiber made shawls and apparels. Because of its scarce availability and lack of fast and reliable techniques to ascertain its origin in textile blends, false declaration of the apparels composition can be made in textile trade and commerce. The rapid development of molecular genetic analysis tools has made it possible to analyze most biological material having nucleic acids intact enough to amplify with PCR. We have developed a method which allows for the reliable detection of the purity of pashmina textiles up to 10% level. A patent for this work has already been filed to Indian Patent Office Patent No- 3400/DEL/2012. Further we have optimized novel primers for amplification of rabbit specific DNA from rabbit hair in textile blends. This developed PCR analytical method can quickly extract total DNA i.e., nuclear DNA (nuDNA) and mitochondrial DNA (mtDNA) from raw or chemically finished animal fibers. Further, we are working to quantify animal origin fibers (sheep, goat, Yak, rabbit, camel and silk) in finished textile products. Such efforts will help to combat adulteration or false declaration and will ensure adherence to the international trading agreements and would provide protection to local manufacturer involved in specialty animal fiber-based cottage industry.

Biography :

Email: rajivbiotech028@gmail.com