Development of a multi-epitope antigen as a serodiagnosis marker of Toxoplasma gondii infection

JOINT EVENT ON 15^th International PHARMACEUTICAL MICROBIOLOGY AND BIOTECHNOLOGY CONFERENCE & 10^th Annual MEDICAL MICROBIOLOGY SUMMIT & EXPO

June 21-23, 2017 London, UK

Abbas Alibakhshi, Mojgan Bandehpour, Zarin Sharifnia and Bahram Kazemi

Beheshti University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Due to the high prevalence of Toxoplasma gondii infection has been attracting a lot of attention to its diagnosis and treatment. The use of pure antigens although in some show high sensitivity and specificity, however, the challenges such as cross-reactivity still remain as diagnostic difficulties. In this study, three surface antigens (SAG) of T. gondii were employed for designing a multi-epitope antigen. The antigen was then expressed using E. coli BL21 (DE3) cells and purified by affinity chromatography. ELISA analysis was used for evaluation of acute toxoplasmosis. The results showed a sensitivity of 72.6% and a specificity of 90.3% for recombinant ELISA.

Biography :

Email: rezasiv@gmail.com