Huiqin Zhuo1, Yi Peng2,3, Qin Yao1, Sufang Zhou2, Xi Li2, Xiaoling Lu1,2,3, Yongxiang Zhao2,3
1Xiaman University, China 2Guangxi Medical University, China 3Guangxi Medical University, China 4Guangxi Medical University, China
Posters-Accepted Abstracts: J Cancer Sci Ther
Targeted gene delivery systems that combine imaging and therapeutic modalities in a single macromolecular construct may offer advantages in the development and application of nanomedicines in vivo. Here, we describe the synthesis, biophysical characterization, tumor cell-selective internalization, and targeted gene delivery system of PEGylated transferrin-conjugated liposomes (PTf-Ls). An improved protocol for DNA encapsulation in interstitial space, based on ethanol/calcium-mediated DNA condensation was developed. The nanoparticles obviously accumulated in tumor cells, exhibited high transfection efficiency, which were resistant to serum, and relatively low cell cytotoxicity. In a Balb/c nude mice breast cancer cells MDAMB- 231-Luc (containing luciferase gene) xenograft model, systemic administration of fluorescence dye (Koadak X-sight 670 Large Stokes Shift Dye) labeled PTf-Ls, which effectively eliminated the nonspecific binding, significantly prolonged circulation, and selectively and efficiently internalized in tumor cells, demonstrated a gradual increase of fluorescence in tumor over time up to 26 hrs, specific tumor imaging at 32 h and lasting for more than 58 hrs. Furthermore, we assessed the utility of this system in cancer gene therapy using Interferon (IFN)-├?┬│ inducible protein-10(IP-10) corded plasmid DNA. It dramatically suppressed tumor growth. Therefore, this new bifunctional PTf-Ls system has the potential for gene delivery and targeted tumor imaging in vivo.