Navreet Kaur Sekhon and Sarabjeet Sharma
Shri Guru Ram Das Institute of Medical Sciences and Research, India
Posters & Accepted Abstracts: J Med Microb Diagn
Tuberculosis(TB), is caused by Mycobacterium tuberculosis. Despite of significant advancement in medical science, it is still one of the leading causes of death and illness worldwide. According to latest WHO global tuberculosis report 2015, approximately 9.6 million people get sick with TB and an estimated 1.6 million died from this disease in 2014 which is even more that HIV/AIDS (1.2 million) (WHO Report, 2015). At present, four first line antibiotics (Rifampicin, Ethambutol, Isoniazid and Pyrazinamide) are being widely used to treat TB under DOTs. Most of the anti TB drugs except streptomycin are reported to cause hepatotoxicity. This is the major cause of discontinuity of DOTs treatment by patients with low income and major factor in emergence of antibiotic resistant strains (MDR and XDR) of M. tuberculosis. Approximately, 60% of the world’s population still relies on medicinal plants for their primary healthcare. Several plant extracts demonstrated antimycobacterium activity. Therefore in the present investigation, we studied the synergistic effect of some plant extracts/phytochemicals along with the Isoniazid and rifampicin. The M. tuberculosis H37Ra was used as model organism. A. vera, A. sativum, A. cepa and A. indica demonstrated the antituberculosis activity. IC50 of antibiotics and plant extracts were determined using resazurine. A. sativum and A. indica demonstrated synergistic effect with isoniazid and rifampicin. Further studies in animal using M. tuberculosis H37Rv are needed to validate these findings.
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