Cytotoxic effects of Doxorubicin on human leukemia Jurkat cells: Drug interactions with quercetin

12^th World Cancer Conference

September 26-28, 2016 London, UK

Vlad Cosoreanu, Ioana Teodora Tofolean, Ramona Babes and Irina Baran

Carol Davila University of Medicine and Pharmacy, Romania

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

The cytotoxic effect of the anticancer drug doxorubicin (DOX) in a human leukemia Jurkat T cell line was determined by flow cytometric assays of cell cycle, apoptosis/necrosis, oxidative status and mitochondrial Ca2+ level. 18-h and 45-h DOX-exposure induced apoptosis with IC50=951 nM, and IC501=135 nM/IC502=1.92 M (bimodal), respectively, which was accompanied by significant oxidative stress generation (IC50=620 nM). The addition of the flavonoid quercetin (QC) (10 �¼M) resulted in a significant decrease of cell viability for DOX levels <100 nM. DOX induced cell cycle arrest displaying a trimodal distribution, so that low, intermediate and high doses of DOX specifically produced G2/M, S and G0/G1 blockage with IC50 of 49 nM, 464 nM and 1866 nM, respectively. QC (15 �¼M) exerted strong antioxidant effects, reducing DOX-induced oxidative stress and apoptosis (IC50=2119 nM and 4897 nM, repectively). However, cell cycle arrest induced by low and moderate doses of DOX was maintained in the presence of QC levels <25 �¼M. DOX induced substantial mitochondrial depolarization within 4-h in a dose-dependent manner (IC50=0.200 nM). A 15-min exposure to DOX induced an immediate decrease of mitochondrial Ca2+ level IC50=18.3 �¼M and the addition of QC (15 �¼M) amplified this effect for a concentration of DOX <20 �¼M but resulted in an increase of mitochondrial Ca2+ for higher concentrations.

Biography :

Vlad Cosoreanu is an undergraduate student of Medicine at Carol Davila University of Medicine and Pharmacy in Bucharest and currently doing research in the Department of Biophysics.

Email: vlad.cosoreanu@yahoo.com