Cytotoxic and anti-inflammatory effects of Pistacia lentiscus leaf extracts

Pharmaceutical Regulatory Affairs: Open Access

ISSN: 2167-7689

Open Access

Cytotoxic and anti-inflammatory effects of Pistacia lentiscus leaf extracts

9th Annual European Pharma Congress

June 26-28, 2017 Madrid, Spain

Djebbar Atmani, Imane Charid, Saliha Remila, Dina Atmani Kilani, Stefanie Schrauwen, Sandra Tuyaerts, Frederic Amant and Jean Louis Connat

University of Bejaia, Algeria
University of Leuven, Belgium
University Hospitals Leuven, Belgium
Antoni van Leeuwenhoek-Netherlands Cancer Institute, Netherlands
University of Bourgogne, France
COHIRO Biotechnology, France

Posters & Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

The present study was carried out to evaluate the anticancer and anti-inflammatory properties of Pistacia lentiscus leaf extracts, a plant widely used in Algerian traditional medication, using various in vitro techniques. The results showed that the crude extracts were not cytotoxic against mammary tumor EMT6 cell lines at all the concentrations tested (25├ó┬?┬?100 ug/ml). However, a relevant cytotoxic activity was noticed against melanoma tumor B16F10 and ovarian cancer cell lines (A2780 and SKOV3) with IC50 values of 56.40 ug/ml, 10 ug/ml and 18 ug/ml, respectively. Moreover, a significant expression in the apoptotic and pre-apoptotic cells A2780 and SKOV3 has been shown after treatment with 10 ug/ml and 25 ug/ml of the crude extract. In contrast, no cell cycle arrest in A2780 cell but an increase in Phase G1 was observed. On the other hand, P. lentiscus extracts induced a significant reduction of pro-inflammatory cytokines (TNF-├?┬▒, IL-1├?┬▓ and IL-6) and a stimulation of the release of anti-inflammatory cytokines (IL-10) in activated macrophage. HPLC and NMR analyses of crude extracts and fractions allowed the identification of many compounds which may be responsible for the observed biological properties. These results may well be the premise for new natural products with chemotherapeutic attributes.

Biography :


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