Naveeda Akhtar Qureshi and Wajiha
Quaid-i-Azam University, Pakistan
Posters & Accepted Abstracts: J Veterinar Sci Techno
Coccidiosis is one of the most economically devastating diseases caused by intracellular protozoan parasite of genus Eimeria. Since last few decades anticoccidial drugs are being used but due to abundant use drug resistance Eimeria strains developed. In the present study three types of egg adapted vaccines namely; egg adapted gametocytes, sonicated gametocytes and formalin inactivated gametocytes has been developed. Comparative efficacy of salinomycin and egg adapted vaccines was investigated. Total of 90 chicks of one day old were divided into six groups (A-F). On 5th and 15th day of age three groups were vaccinated orally with 0.2 ml vaccines. On 21st day of age all groups were given infection with dose of 10000 oocysts per bird except control group. On 5th day post inoculation 60 mg/kg salinomycin was given to unvaccinated medicated group to check the comparative effectiveness of vaccines and drug. Gametocytes vaccinated and salinomycin treated group had significantly higher (P├ó┬?┬Ą0.002) body weight gain, feed consumption and mild bloody diarrhea when compared with formalin and sonicated vaccinated and infected non-medicated unvaccinated groups respectively. Results of biochemical tests showed significant decrease in uric acid, creatinine, AST, ALT and increase level of ALP and albumin in all groups except positive control. Histopathological examination showed Eimeria induced lesion were mild in gametocytes vaccinated, medicated and control group. While in non-immunized infected group the lesions were severe. Indirect hemagglutination test was applied for detection of antibodies in vaccinated groups and maximum in gametocytes vaccinated group. It is concluded that live gametocytes vaccine were found to be more effective against coccidiosis and less virulent than sporocytes which were reported previously to be more virulent than gametocytes.
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