Chromosome-based survey sequencing of Triticum dicoccoides

9^th International Conference on Genomics & Pharmacogenomics

June 15-16, 2017 London, UK

Bala Ani Akpinar, Burcu Alptekin, Miroslava Havrankova, Jan Vrana, Jaroslav Dolezel, Assaf Distelfeld, Pilar Hernandez and Hikmet Budak

Sabanci University Nanotechnology Research and Application Centre, Turkey

Posters & Accepted Abstracts: J Mol Genet Med

Abstract :

Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of tetraploid durum and bread wheat. With its direct ancestry to modern wheat cultivars and its rich gene pool carrying favorable alleles for grain quality and stress tolerance, T. dicoccoides is a promising source for wheat improvement. Here, we report a chromosome-based insight into its entire genome, through the survey sequencing of all its 14 chromosomes. Genomic scaffolds constructed by aligning de novo assembled contigs to the recently assembled genome of Zavitan genotype and delivered important clues into the T. dicoccoides genome, unraveling coding regions including flanking regulatory sequences for a number of agronomically important genes. Repetitive element annotations revealed considerable differences in content and distribution in A- and B-genome chromosomes. Gene contents of the chromosomes varied widely, not necessarily correlating with chromosome sizes. Syntenic relationships and virtual gene orders indicated several small-scale rearrangements, in addition to, providing evidence to the 4AL-5AL-7BS translocation in wild emmer wheat at the sequence level. Chromosome-based sequence assemblies also revealed 7 novel miRNA families, of a total of 47 putatively encoded genes by the entire genome. The survey sequences from all 14 chromosomes of T. dicoccoides not only shed light into its genome, but also provided a large-scale, much needed additional resource for the discovery of potentially new alleles and design of novel molecular markers that should benefit fellow researchers and breeders.

Biography :

Email: aniakpinar@sabanciuniv.edu