Characterization of mutations causing rifampicin and isoniazid resistance of Mycobacterium tuberculosis

2^nd International Conference on Respiratory and Pulmonary Medicine

October 17-18, 2016 Chicago, USA

Maya Habous

Rashid Hospital-Dubai Health Authority, UAE

Posters & Accepted Abstracts: J Pulm Respir Med

Abstract :

Objective: To characterize mutations causing rifampicin and isoniazid resistance of M. tuberculosis in Syria. Methods: 69 rifampicin resistant (RIFr) and 72 isoniazid resistant (INHr) isolates were screened for point mutations in hot spots of the rpoB, katG and inhA genes by DNA sequencing and real time PCR. Results: Of the 69 RIFr isolates, 62 (90%) had mutations in the rifampin resistance determining region (RRDR) of the rpoB gene, with codons 531 (61%), 526 (13%) and 516 (8.7%) being the most commonly mutated. We found two new mutations (Asp516Thr and Ser531Gly) described for the first time in the rpoB-RRDR in association with rifampicin resistance. Only one mutation (Ile572Phe) was found outside the rpoB-RRDR. Of 72 INHr strains, 30 (41.6%) had a mutation in katG codon 315 (with Ser315Thr being the predominant alteration), and 23 (32%) harbored the inhA�15C

Biography :

Email: dr.mayah@yahoo.com