Chiraz Soumia Amrine
Accepted Abstracts: Altern Integ Med
Natural products continue to be an important source of unique and new chemical entities in drug research and development. Eight withanolides were isolated from the leaves of Withania obtusifolia Taeckh. (Solanaceae). This Jordanian species is closely related to Withania somnifera , the most important and most studied of Withania species known as Ashwagandha or Indian Ginseng. Ethanolic extract was purified using different chromatographic techniques (open silica gel columns, dextran columns, preparative TLC and semi-preparative HPLC). Of the isolated compounds, two were new [obtusifonolide (1) and 27-glucoisowithaferin A (2)], four were new to the species [ 6α -chloro- 5β -hydroxy withaferin A (3), isowithanone (4), 3-ethoxy-2,3-dihydroxy withaferin A (5), and daturataturin A (6)], and two were reported previously from the same species [withaferin A (7) and 4α, 27-dihydroxy -1-oxo- 5β, 6β-epoxy witha -2, 24-dienolide (8)]. The structures were elucidated using a set of spectroscopic and spectrometric techniques principally: MS (APCI and ESI), 1D-NMR ( 1 H and 13 C) and 2D-NMR (COSY, HMBC, HMQC and NOESY). Compounds (1-8) were evaluated for cytotoxicity against a human cancer cell panel and for antimicrobial activity in an array of bacteria and fungi. In the cell lines tested, compounds 7 and 8 were the most potent with IC 50 values ranging from 0.30 to 1.7 μM.
Chiraz Soumia Amrine is an Algerian lecturer and researcher at the University of Saad Dahleb-Blida (Algeria). She has received her M.Sc. degree in Natural Products Chemistry from Jordan University of science and technology (Jordan), her great interest in phytochemistry and bioactive natural compounds has let her to be part of some researches on medicinal and aromatic plants.
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