Alina Romanenko, Sergey Vozianov, Vyacheslav Grygorenko, Anna Chekalova, Andriy Yurakh and Pirkko Harkonen
Accepted Abstracts: J Cancer Sci Ther
During the 26-years period subsequent to the Chernobyl accident the morbidity of prostate cancer in Ukraine has increased from 12.0 to 28.6 per 100 000 of male population. The present study was conducted to evaluate the development of radiation- dependent lesions in benign prostate hyperplasia (BPH) patients living in cesium 137 ( 137 Cs) contaminated areas of Ukraine. BPH samples were obtained by adenomectomy from 30 patients from so called clean (without radio-contamination) areas (control group 1) and 90 patients living in 137 Cs contaminated areas of Ukraine (group 2). These BPH samples were examined histologically and immunohistochemically (IHC). γ-H2AX, iNOS, Ki-67, p53, p63, p27Kip1 and Bcl-2 proteins were IHC investigated in BPH samples from all patients. A pattern of chronic proliferative atypical prostatitis (CPAP) accompanied with large areas of sclerotic stromal connective tissue with increased angiogenesis, in association with dramatic increase in the incidences of areas of proliferative inflammatory atrophy (PIA), basal-cell hyperplasia (BCH) with cellular atypia as well as with the areas of prostatic intraepithelial neoplasia (PIN) were detected in group 2 BPH patients. Our data support a strong relationship between long-term low-dose 137 Cs radiation exposure of BPH patients who lived about 26 years in radio contaminated areas and development of CPAP, a possible preneoplastic condition in humans. Our study suggests the alteration of cell cycle transition and apoptotic regulatory molecules in association with γ-H2AX and iNOS over expression at the areas of PIA and BCH with cellular atypia which could be also crucial early molecular events in the pathogenesis of the radiation induced prostate carcinogenesis.