Anti-inflammatory proteins in meconium

6^th International Conference on Biomarkers & Clinical Research

August 31-September 02, 2015 Toronto, Canada

Ewa Skar�¼y��ska, Barbara Lisowska-Myjak and Paulina Jankowska

Medical University of Warsaw, Poland

Posters-Accepted Abstracts: J Mol Biomark Diagn

Abstract :

Levels of faecal proteins above cutoffs are of proven diagnostic value. Examples include proteins secreted by neutrophils in inflammatory focus in the intestine such: Calprotectin (Cal) from the cytoplasm and lactoferrin (LF) from specific granules. Another protein, ceruloplasmin (CP) is produced by hepatocytes and activated monocytes and macrophages. The aim of study was to evaluate the occurrence of three proteins in meconium, a specific type of faeces formed in the fetal intestine from 12 weeks gestation and excreted in the first 48 hours after birth. The protein concentrations were measured in meconium homogenates using commercial ELISA kits. Meconium from 20 neonates was assessed. The number of meconium portions n=81. The weight of a single meconium portion [g]: Range=0.18-18.93, mean�±SD=5.52�±4.02. The concentrations measured were as follows [�¼g/g]: Cal, range=33.8-1067.1, mean�±SD=286.5�±214.6; LF, range=1.7-511.4, mean�±SD=45.1�±78.5; CP, range=52.2�±1076.0, mean�±SD=310.6�±228.9. A correlation was between LF-CP (r=0.46, p<0.0001) no correlation was found between Cal-LF (r=0.3, p=0.19) and Cal-CP (r=0.16, p=0.15). The weight of meconium filling the fetal intestine [g]: Range=4.72-36.95, mean�±SD=18.29�±8.64. The sum content of each protein in all meconium portions passed by a neonate was considered to equal the amount of the protein accumulated. The amounts were as follows [mg]: Cal, range=1.2-6.3, mean�±SD=3.4�±1.4; LF, range=0.021-2.7, mean�±SD=0.76�±0.75; CP, range=0.79-17.2, mean�±SD=4.7�±4.34. The occurrence of markers for intestinal inflammation from start of intrauterine life, at levels above cutoffs requires further studies. The proteins concentrations measured in this study were varied between meconium portions from one neonate. There were also significant differences in total accumulation of proteins between individual neonates.

Biography :

Ewa Skar�¼y��ska has completed her PhD from Medical University of Warsaw, Faculty of Pharmacy, Department of Biochemistry and Clinical Chemistry. She is responsible for teaching at graduate and postgraduate levels.

Email: ewaskarzynska@wp.pl