A comparative study on the efficacy of conventional diagnostic methods vs PCR in the detection of Helicobacter pylori Infection amongst chronic gastritis patients of Anand region

3^rd International Conference on Diagnostic Microbiology and Infectious Diseases

September 24-25, 2018 | Montreal, Canada

Jayeshkumar Ruparelia and Milankumar Pathak

SP University, India

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Introduction: Helicobacter pylori is currently implicated in the pathogenesis of various gastric and duodenal disorders and also a risk factor in gastric carcinomas. H. pylori infection is detected by various invasive & noninvasive methods and each method has its own pits & falls. Out of all the techniques PCR is considered to be the most rapid, accurate and sensitive method. Purpose: The present study is an attempt to evaluate and compare the efficiency of conventional diagnostic techniques with PCR. Methods: Four fragments of antral biopsies were collected from 100 patients & were processed for PCR, RUT, Culture and Gram staining. A serum sample was processed for determination of IgG antibodies (indirect ELISA). Results: The detection rate H. pylori infection of various tests was as follows serology 54%, PCR 44%, RUT 36%, Gram staining 24% and Culture 22%. The sensitivity of Serology and PCR was found higher at 100% than RUT (81.81%), Gramâ??s staining (54.54%) and culture (50%). The specificity of PCR, RUT, Gramâ??s staining and culture were 100% and serology was 82.14%. Among the five diagnostic methods PCR was most accurate with 100% accuracy and the highest agreement of PCR was found with RUT. Conclusions: Our study suggests that association of PCR & Serology constitutes the best choice for confirming the diagnosis due to its high concordance rate followed by RUT and serology if PCR is not possible due to limited resources.

Biography :

E-mail: jayurupareliya@gmail.com