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Epilepsy Journal

Epilepsy Journal

ISSN: 2472-0895

Open Access
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MicroRNA Profiling Journals

MicroRNA  regulate gene expression through posttranscriptional mRNA degradation or suppression of translation. Many analytical issues remain to be resolved for miRNA screening with TaqMan Low Density Arrays  in plasma samples, such as optimal RNA isolation, preamplification and data normalization. We optimized the TLDA protocol using three RNA isolation protocols and preamplification dilutions. By using 100μL elution volume during RNA isolation and adding a preamplification step without dilution,  of wells were amplified. Informative target miRNA were defined as having quantification cycle values of samples and low technical variability  A total of 218 miRNA was considered informative. Different normalization strategies were compared: exogenous Ath-miR-159a, endogenous RNA U6

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Citations: 41

Epilepsy Journal received 41 citations as per Google Scholar report

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