Medical Microbiology & Diagnosis

ISSN: 2161-0703

Open Access

Mehiri Emna

Department of Microbiology, Hospital Abder-Rahmenmami of Pneumo-Physiology, Ariana, Tunisia

  • Research Article   
    Diagnosis of Pneumocystis jirovecii Pneumonia by Real Time PCR: An Analysis of 10 Clinical Cases
    Author(s): Baccouchi Nawel*, Mtibaa Latifa, Souid Hana, Mehiri Emna, Slim Leila and Jemli Boutheina

    Introduction: Pneumocystis jirovecii Pneumonia (PCP) is a life-threatening disease in immunocompromised patients. The aim of this work is to demonstrate the contribution of molecular biology in diagnosis compared to conventional methods and we propose an adapted cut-off value for differentiating Pneumocystis colonization from infection using real-time PCR. Methodology: This was a prospective study enrolled from April 2015 to December 2018 at the Laboratory of parasitology of Military Hospital of Tunis. All pulmonary secretions samples were analyzed using: May-Grunwald-Giemsa (MGG) and Gomori Grocott Modified MUSTO Coloring Technology (GG). Optimization of conventional PCR and real-time PCR were accomplished. Results: During the study period, we collected 200 samples. The prevalence of the diseas.. Read More»

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