Ethiopia						                            
                            
						
 Research Article
												Investigation of Newcastle Disease Virus Using Reverse Transcription Polymerase Chain Reaction in Selected Districts of Eastern Shewa, Ethiopia 						
Author(s): Yemsrach  Miressa, Yoseph  Cherinet, Abrha  Bsrat and Fanos  TadesseYemsrach  Miressa, Yoseph  Cherinet, Abrha  Bsrat and Fanos  Tadesse             
						
												
				 In Ethiopia Newcastle disease virus is the most important cause of loss in village-dwelling as well as commercially raised chickens. The disease occurs almost at any time of a year throughout the country including East Shewa Zone. A cross sectional study was therefore conducted from December 2014 to May 2015 to determine the prevalence of Newcastle disease in market and village chicken in selected districts of East Shewa zone of Ethiopia. Molecular diagnostic technique, Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was employed to detect the viruses during the study. A total of 300 swab samples was collected using simple random sampling technique in Adama and Bishoftu Districts. An overall prevalence of 26.7% (40/300) was recorded during this study from the collected swab samples using real time PCR. The result of this study indicated that village chicken flock are endemica.. Read More»
				  
												DOI:
												 10.4172/2157-7579.1000384 
																	  
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