Molecular and Genetic Medicine

ISSN: 1747-0862

Open Access

Thorsten Bergmann


  • Review Article
    Progress and Problems with Viral Vectors for Delivery of Talens
    Author(s): Thorsten Bergmann, Eric Schulz and Anja EhrhardtThorsten Bergmann, Eric Schulz and Anja Ehrhardt

    It has long been envisaged that gene disruption or gene correction in affected target cells can be efficiently conducted in vitro and in vivo and over the recent years several tools for achieving this goal were developed. Designer nucleases such as zinc finger nucleases (ZFNs) were extensively explored and more recently transcription activator-like effector nucleases (TALENs) were introduced for sequence-specific genome engineering in the mammalian genome. ZFNs and TALENs are fusion proteins containing a customized DNA-binding motif for sequence-specific DNA binding linked to a nuclease for introduction of double-stranded DNA breaks. Both systems were explored in mammalian cells using non-viral and viral delivery methods. Herein, we will provide a state-ofthe- art overview of available virus-based delivery systems for sufficient expression of functional TALENs. We will cover the molec.. Read More»

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