Journal of Cytology & Histology

Silverio Sbrana

Publications

• Research Article
  Phenotype Changes of Circulating Monocytes in a Hypercholesterolemic Swine Model of Coronary Artery Disease
  Author(s): Silverio Sbrana, Gualtiero Pelosi, Maria Rita Puntoni, Federica Viglione, Maria Giovanna Trivella and Oberdan ParodiSilverio Sbrana, Gualtiero Pelosi, Maria Rita Puntoni, Federica Viglione, Maria Giovanna Trivella and Oberdan Parodi
  
  Objective: Inflammation and immunity activation play a key role in atherosclerosis (ATS) onset and progression. Aim of this study was to investigate the relationships between phenotype of circulating monocytes and coronary artery disease (CAD) development in a histologically well-characterized swine model of ATS. Methods: Blood samples were obtained from 6 animals at baseline and after 16 weeks high fat cholesterolenriched diet. Flow cytometry monocyte identification was performed (CD172a marker). Adhesion (CD18a, CD11a, CD11R3, CD49d, CD29), differentiation (CD14) and activation receptors (SLA-DR, CD16, CD163) were quantified as percentage of positivity (%) and Relative Fluorescence Intensity (RFI). Lipid parameters (LDL, oxLDL, HDL) and soluble endothelial ICAM-1 were measured and histologic quantitative assessment of coronary ATS was perfor.. Read More»
  DOI: 10.4172/2157-7099.1000270

  Abstract PDF