Journal of Tissue Science and Engineering

Silva ARP

Publications

• Research Article
  Development of a Protocol for Human Adipose Stem Cell Culture in CO₂ Independent Medium and Perfusion Bioreactor
  Author(s): Silva ARP, Paula ACC, Zonari A, Martins TMM, A M Goes and M M PereiraSilva ARP, Paula ACC, Zonari A, Martins TMM, A M Goes and M M Pereira
  
  Advances in research on stem cells derived from human Adipose tissue (hASC) may allow its use for bone tissue engineering. In such context, it is important to standardize a methodology to culture cells in high quantity. Bioreactors, in which cells are cultured in three-dimension, mimic the physiological environment in vitro, allowing the hASC maintenance, proliferation and differentiation. The aim of the study was to assemble a perfusion bioreactor, and to develop a new protocol for hASC cultivation in a CO2 independent medium to be used in the bioreactor. The hASC were isolated from human lipoaspirate, and a two-dimensional cell culture was performed in DMEM, supplemented with 10% Fetal Bovine Serum (FBS). The follow step was to adapt the cells to Leibovitz’s CO2 Independent Medium (LEI+10% FBS), and compare the MTT and Alkaline Phosphatase Activity (ALP) assays to cells .. Read More»
  DOI: 10.4172/2157-7552.S11-006

  Abstract PDF