Journal of Molecular Biomarkers & Diagnosis

Samiah Yasmin Abdul Kadir

Publications

• Research Article
  Protein Map Standardization of Human Saliva Using Two Dimensional Gel Electrophoresis (2-DE)
  Author(s): Saeid Reza Doustjalali, Ali Yaldrum, Karim Al- Jashamy, Mohammed Irfan, Khin Thant Zin, Nyan Htain Linn, Wai Ma Lin, Vinothini Appalanaidu, Samiah Yasmin Abdul Kadir, Jeyaseelan Nadankutty, Rohaini Mohamad, Wong A- Chin, Htet Htet, Ahmad Yusuf, Rebecca SY Wong, Vinoth Kumarasamy, Christinal PW Teh, Nazrila SF Suhaimi, Hafiza Arzuman, Aida Nur Ashikin Abdul Rahman, Marzalina Mansor, Shamala Devi Sekaran and Negar Shafiei SabetSaeid Reza Doustjalali, Ali Yaldrum, Karim Al- Jashamy, Mohammed Irfan, Khin Thant Zin, Nyan Htain Linn, Wai Ma Lin, Vinothini Appalanaidu, Samiah Yasmin Abdul Kadir, Jeyaseelan Nadankutty, Rohaini Mohamad, Wong A- Chin, Htet Htet, Ahmad Yusuf, Rebecca SY Wong, Vinoth Kumarasamy, Christinal PW Teh, Nazrila SF Suhaimi, Hafiza Arzuman, Aida Nur Ashikin Abdul Rahman, Marzalina Mansor, Shamala Devi Sekaran and Negar Shafiei Sabet
  
  Over the past decade, the use of saliva as an auxiliary diagnostic tool for biomarker detection has gained considerable acceptance as a non-invasive, inexpensive alternative to conventional serum method. In proteomics, the most promising technique which can be used for detecting salivary biomarker with sufficient resolving power is two-dimensional gel electrophoresis (2-DE) that provides a unique platform for the simultaneous separation of proteins in a complex mixture. However, as a fact most of the new scientists in developing countries are still facing problems with optimization of 2-DE techniques because the performance of optimization techniques in proteomics research using 2-DE has its own limitations. Therefore, the present study was established to generate a reproducible and optimized protocol to display the 2-DE protein map of human saliva. Our results showed the standard opt.. Read More»
  DOI: 10.4172/2155-9929.1000249

  Abstract PDF