Tanzania
Research Article
A Novel Method for Mutation Analysis Using Genomic DNA Obtained from Immunohistochemistry-Stained Sections
Author(s): Rupal Desai, Rajesh Patel, Lukas Amler, Hartmut Koeppen, Ian McCaffery, Gianni Luca, Astrid Kiermaier and Rajiv RajaRupal Desai, Rajesh Patel, Lukas Amler, Hartmut Koeppen, Ian McCaffery, Gianni Luca, Astrid Kiermaier and Rajiv Raja
Background: Tumor biopsies obtained from patients are often limited in size and availability, and the ability to perform multiple diagnostic assays depends on the quantity and quality of the tissue. Here we describe and evaluate a method for performing DNA-based mutational analyses after immunohistochemistry analysis has been performed, using a single tissue section.
Method: Immunohistochemistry analysis was performed on 4-5 μm formalin-fixed paraffin-embedded tumor tissue sections and immunohistochemistry-stained sections were stored for subsequent genomic analysis. DNA was isolated from these immunohistochemistry-stained sections and DNA quality was assessed using a multiplexpolymerase chain reaction method as well as real time quantitative polymerase chain reaction of commonly used reference genes. Subsequently, genomic DNA was pre-amp.. Read More»
DOI:
10.4172/2155-9929.1000228
Journal of Molecular Biomarkers & Diagnosis received 1874 citations as per Google Scholar report
