Journal of Tissue Science and Engineering

Ruhma Mahmood

Publications

• Research Article
  Pre-Differentiated Skin-Like Cells can Alleviate In Vitro Burn Injury
  Author(s): Ruhma Mahmood, Mahmood S Choudhery, Shaheen N Khan and Sheikh RiazuddinRuhma Mahmood, Mahmood S Choudhery, Shaheen N Khan and Sheikh Riazuddin
  
  Considering importance of stem cell based therapies for wound healing, we have previously established protocols for successful differentiation of stem cells (isolated from placenta) into skin-like cells (keratinocytes and fibroblasts). In the current study we aim to evaluate the effect of pre-differentiated skin-like-cells on wound healing potential using an in vitro burn injury model. The amniotic epithelial cells (AECs) and umbilical cord mesenchymal stem cells (UCMSCs) obtained from amniotic membrane and umbilical cord tissue; respectively, were differentiated into skin-like cells (keratinocyte and fibroblasts respectively). In order to make an in vitro burn injury model, keratinocytes and fibroblasts were isolated from rats and insulted with high temperature (up to 50oC). The optimal temperature for burn injury models was determined using viability assay, cytotoxicity level, proli.. Read More»
  DOI: 10.4172/2157-7552.1000189

  Abstract PDF