Veterinary Science & Technology

Raphael Chukwujekwu Kene

Publications

• Research Article
  Use of Native Bovine Bone Morphogenetic Protein Extract in Healing Segmental Tibial Bone Defects in Goats
  Author(s): Edwin Aihanuwa Uwagie, Augustine Cheh Awasum, Raphael Chukwujekwu Kene and Ferdinand Chiemeke ChilakaEdwin Aihanuwa Uwagie, Augustine Cheh Awasum, Raphael Chukwujekwu Kene and Ferdinand Chiemeke Chilaka
  
  We tested the new bone-forming activity of injectable native bovine BMP extracted from cadaveric bones obtained from the abattoir after SDS PAGE electrophoresis was used to determine the molecular weights and extract was bio-assayed in the goat thigh muscle pouch model. The bovine BMP extract was further used as an implant in an absorbable collagen sponge (ACS) and hydroxyapatite to heal segmental bone defects in a large animal tibia fracture model. Open tibia fractures were created in 20 adult goats with loss of 1.5 cm segment of the bone. 10 goats were treated with an implant of the study device (0.2 mg of extracted bovine BMP, ACS and hydroxyapatite) while 10 goats were treated with an implant of Buffer, ACS and hydroxyapatite. The devices were implanted as a mould in the segmental defects. The animals were monitored for callus formation which was measured on lateral radiographs an.. Read More»
  DOI: 10.4172/2157-7579.1000329

  Abstract PDF