Journal of Clinical & Medical Genomics

Pradeep K Chatterjee

Publications

• Review Article
  Trapping Enhancers by Transgenic Expression of BACs Engineered in Bacteria with loxP Transposons
  Author(s): Hope M Wolf, Kevin O Nyabera, Katya K De La Torre, Mugtaba A Eltayeb, Leighcraft A Shakes, Charles Hatcher, Derek C Norford and Pradeep K ChatterjeeHope M Wolf, Kevin O Nyabera, Katya K De La Torre, Mugtaba A Eltayeb, Leighcraft A Shakes, Charles Hatcher, Derek C Norford and Pradeep K Chatterjee
  
  Bacterial Artificial Chromosomes (BACs) are large extra-chromosomal plasmids in bacteria that faithfully propagate large pieces of DNA from the chromosomes of organisms. Because they represent tiny contiguous pieces of the chromosome, BACs are ideally suited for expression of genes in their chromosomal contexts. Genes in BACs can be monitored for expression after the DNA is modified with reporter genes and other sequences that allow it to be stably propagated in the new host. Several methods have been developed to alter BAC DNA within its bacterial host. One approach uses Tn10 mini-transposons to introduce exogenous DNA into BACs. The random insertions of Tn10 carrying lox sites have directed mammalian cell-selectable antibiotic resistance genes, enhancer-traps and inverted repeat ends of the vertebrate transposon Tol2 precisely to the ends of genomic DNA inserts in BACs. Reporter gen.. Read More»
  DOI: 10.4172/2332-0672.1000114

  Abstract PDF