Journal of Cytology & Histology

Michele Diana

Publications

• Research Article
  Functional Capillary Density for in Vivo Estimation of Intestinal Perfusion using Real-Time Confocal Endomicroscopy
  Author(s): Luigi Schiraldi, Francesco Marchegiani, Michele Diana, Véronique Lindner, Eric Noll, Pierre Diemunsch and Jacques MarescauxLuigi Schiraldi, Francesco Marchegiani, Michele Diana, Véronique Lindner, Eric Noll, Pierre Diemunsch and Jacques Marescaux
  
  Background and aim: Confocal Laser Endomicroscopy (CLE) has been successfully used to appreciate microcirculation changes of the digestive mucosa. Our aim was to evaluate CLE scanning complemented by functional capillary density area (FCD-A) estimation to define the micro-vessel status in a reiterate, long-lasting porcine model of bowel ischemia. Materials and methods: A laparotomy was performed in 4 pigs, and a segmental (3–4 cm) ischemia of the sigmoid colon was induced with vascular clamps. Ischemic and perfused regions were clinically defined. After an injection of 5 ml of sodium fluorescein 10% (Fluocyne, SERB, Paris, France), the Cellvizio™ confocal probe (Mauna Kea Technologies, Paris, France) was directly applied onto the mucosa’s surface through a full-thickness enterotomy. Both ischemic area (IA) and control region-.. Read More»
  DOI: 10.4172/2157-7099.1000334

  Abstract PDF