Veterinary Science & Technology

Mahipal Singh

Publications

• Research Article
  Recovery of Fibroblast-Like Cells after 160 Days of Postmortem Storage of Goat Skin Tissues in Refrigerated Media
  Author(s): Hadj S Aoued and Mahipal SinghHadj S Aoued and Mahipal Singh
  
  Animals have been cloned from frozen decade old postmortem tissues preserved within few hours of animal death. Delay in tissue preservation may reduce cloning success due to compromised nuclear DNA integrity. In vitro culture of cells ensures nuclear integrity and is a preferred method of preparing somatic cells for cloning. However, the time limits of postmortem recovery of cells capable of in vitro culture are not precisely known. Here we show recovery of fibroblast-like cells after 160 days of postmortem storage of goat skin in culture media at 4?C. Forty skin explants were cultured at 10 days interval up to 160 days and the outgrowing fibroblast-like cells around them were observed under inverted microscope. Explants with a cluster of more than 50 cells, after 10-12 days of culture initiation, were considered positive. We observed the outgrowth in all the time points, however, the.. Read More»
  DOI: 10.4172/2157-7579.1000236

  Abstract PDF