College of Medicine, Mayo Clinic,
200 First Street SW, Rochester, MN 55905
Tanzania
Research Article
Methylated Bone Morphogenetic Protein 3 (BMP3) Gene: Evaluation of Tumor Suppressor Function and Biomarker Potential in Biliary Cancer
Author(s): John B Kisiel, Jia Li, Hongzhi Zou, Abdul M Oseini, Benjamin B Strauss, Kadra H. Gulaid, Catherine D Moser, Ileana Aderca, David A Ahlquist, Lewis R Roberts and Abdirashid M ShireJohn B Kisiel, Jia Li, Hongzhi Zou, Abdul M Oseini, Benjamin B Strauss, Kadra H. Gulaid, Catherine D Moser, Ileana Aderca, David A Ahlquist, Lewis R Roberts and Abdirashid M Shire
Background: Although cholangiocarcinoma (CC) is an uncommon and highly lethal malignancy, early detection enables the application of potentially curative therapies and improves survival. Consequently, tools to improve the early diagnosis of CC are urgently needed. During a screen for genes epigenetically suppressed by methylation in CC that might serve as methylation markers for CC, we found that the BMP3 gene is methylated in CC cell lines, but the potential diagnostic value and the function of BMP3 in CC are unknown.
Methods: We aimed to quantitatively assess BMP3 methylation in resected CC tumor specimens using methylation specific PCR and evaluate the tumor suppressor role of BMP3 in biliary cancer cell lines in comparison to an immortalized normal cholangiocyte cell line. Expression of BMP3 was quantified by mRNA levels before and after t.. Read More»
DOI:
10.4172/2155-9929.1000145
Journal of Molecular Biomarkers & Diagnosis received 1874 citations as per Google Scholar report
