Journal of Tissue Science and Engineering

Kazuhiro Misumi

Publications

• Research Article
  Osteochondral Regeneration of the Loading-bearing Site Using a Scaffold Free Three-dimensional Construct of Adipose Tissue-derived Mesenchymal Stem Cells in Pigs
  Author(s): Daiki Murata, Satoshi Tokunaga, Shizuka Akieda, Koichi Nakayama, Kentaro Setoyama, Makoto Fujiki and Kazuhiro MisumiDaiki Murata, Satoshi Tokunaga, Shizuka Akieda, Koichi Nakayama, Kentaro Setoyama, Makoto Fujiki and Kazuhiro Misumi
  
  Background: Many surgical strategies for reconstruction of both bone and cartilage have ever been investigated to restore joint structure and function in the late stages of Osteoarthritis (OA). This study was designed to investigate the regeneration of articular cartilage and subchondral bone in the loading-bearing site using a three-dimensional (3D) construct of autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs). Methods: A 3D construct consisting of approximately 1,920 spheroids each containing 5.0 × 104 AT-MSCs was implanted into an osteochondral defect (with a diameter of 6.8 mm and a depth of 6 mm) in the right femoral medial condyle in five adult mini-pigs. The contralateral (left femoral) defect was the control. At three and six months post-operatively, the defects were evaluated using both CT and MR imaging. The radiolucent volume (RV, mm3) of the .. Read More»
  DOI: 10.4172/2157-7552.1000165

  Abstract PDF