Journal of Tissue Science and Engineering

Hiroshi Ohtake

Publications

• Research Article
  Surface-Bound Vascular Endothelial Growth Factor Promotes Prolonged Activation of Endothelial Cells: A New Technology for Capturing Endothelial Progenitor Cells by Intravascular Stents
  Author(s): Shotoku Tagawa, Takehisa Matsuda, Tatsuki Aomizu, Makoto Kuwana, Hiroshi Ohtake, Go Watanabe and Masakazu YamagishiShotoku Tagawa, Takehisa Matsuda, Tatsuki Aomizu, Makoto Kuwana, Hiroshi Ohtake, Go Watanabe and Masakazu Yamagishi
  
  In -situ selective capture of the Endothelial Progenitor Cell (EPC) in the arterial blood stream can provide antithrombogenic and -cell proliferation potential to implanted intravascular stents. Therefore, we defined molecular mechanisms of EPC activation associated with stent surface-bound proteins. We sought suitable bound protein to capture and proliferate EPCs. Then, to determine whether and how long the surface-bound protein activates intracellular signal-transduction pathways of endothelial cells through its receptor, we studied the phosphorylation of key intracellular macromolecules including Vascular Endothelial Growth Factor (VEGF) Receptor (R)-2 (VEGFR-2), focal adhesion protein-tyrosine kinase, Akt, and extracellular signal-regulated kinase in human umbilical vein endothelial cells. We found the most suitable surface-bound protein was VEGF... Read More»
  DOI: 10.4172/2157-7552.1000140

  Abstract PDF